WizPure™ qPCR Master (EVA) is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen dye. It comprises all the components necessary to perform qPCR: WizPure™ HS-Taq DNA Polymerase, ultrapure dNTPs, MgCl2, and EvaGreen dye. The user simply needs to add water, a template, and primers. Hot start DNA Polymerase is activated by a 5-minute incubation step at 95°C. This prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. The kit includes the components necessary for performing PCR amplification and has been successfully used to amplify and detect various DNA targets such as genomic DNA, cDNA, and plasmid DNA.