• Enzymes > DNA Polymerase

    WizPure™ Pfu DNA Polymerase

    Technical Data Image
    WizPure™ Pfu DNA Polymerase
    WizPure™ Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5'→3' direction. Pfu DNA Polymerase also possesses 3'→5'exonuclease (proofreading) activity. WizPure™ Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, and is even up to tenfold more accurate than normal Taq DNA polymerase. Consequently, WizPure™ Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.
    Cat No. Size Remark
    W1331E 500 U not included dNTP
    W1331E-5 2,500 U not included dNTP
    W1331 500 U included dNTP
    W1331-5 2,500 U included dNTP

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    • Overview
      • Error rate : 1.3 x 10-6
      • Time to 1kb (72°C): 60-120
      • Thermal stability: Half-life of 4 hrs at 95°C
      • Blunt end products
      • Recombinant proteins expressed in E. coli
      • Free of detectable non-specific nuclease
      • High-purity enzyme completely free of E.coli DNA contamination
    • Technical Data
      Technical Image
    • Application
      • High fidelity PCR for cloning
      • Amplification of long DNA fragments (>5 - 15 kb)
      • DNA amplification to generate blunt end products
      • Site-directed mutagenesis
      • DNA sequencing
    • Storage & Shipping
      • Store at -20ºC in a frost-free freezer
      • Shipped on ice packs and/or dry ice