• Enzymes > W2301

    WizPure™ MMLV RTase (RNase H+)

    Technical Data Image
    WizPure™ MMLV RTase (RNase H+)
    WizPure™ MMLV RTase (RNase H+), encoded by Moloney Murine Leukemia Virus (MMLV), is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. This enzyme can synthesize a complementary DNA strand, initiating from a primer using RNA or DNA templates. MMLV RTase will also extend primers hybridized to single-stranded DNA. Second-strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.
    Cat No. Size Concentration Remark
    W2301 10,000 U 200U/μl 1 x 50μl, MMLV RTase (RNase H+) 1 x 500μl, 5X Reaction Buffer
    W2301-5 50,000 U 200U/μl 1 x 250μl, MMLV RTase (RNase H+) 1 x 2,500μl, 5X Reaction Buffer

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    • Overview
      • 5' to 3' exonuclease: No
      • 3' to 5' exonuclease: No
      • 3’ – A Overhang: No
      • Fragment Size: Up to 10 kb
      • Free of endo- and exodeoxyribonucleases, phosphatases, and ribonuclease
      • 200 units/μl
      • High-purity enzyme completely free of E.coli DNA contamination
      • GMP grade
    • Technical Data
      Technical Image
    • Application
      • Gene synthesis
      • First-strand synthesis of cDNA
      • RT-PCR
      • Random priming reactions
      • Library construction
      • Probe labeling
      • mRNA 5’-end mapping
    • Storage & Shipping
      • Store at -20ºC in a frost-free freezer
      • Shipped on ice packs and/or dry ice