• Enzymes > W2303

    WizScript™ III Reverse Transcriptase

    Technical Data Image
    WizScript™ III Reverse Transcriptase
    WizScript™ Reverse Transcriptase is a genetically modified M-MLV Reverse Transcriptase that exhibits RNA or DNA-dependent DNA polymerase but lacks ribonuclease H activity. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase in full-length cDNA products. The RNase H activity degrades the RNA part of the cDNA/RNA hybrid following reverse transcription, which may prevent its inhibition during subsequent PCR steps.
    Cat No. Size Concentration Remark
    W2303 10,000 U 200U/μl 1 x 50μl, WizScript™ III Reverse Transcriptase 1 x 500μl, 5X Reaction Buffer
    W2303-5 50,000 U 200U/μl 5 x 50μl, WizScript™ III Reverse Transcriptase 5 x 500μl, 5X Reaction Buffer

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    • Overview
      • RNase H negative
      • Molecular weight: 71 kDa
      • Reaction temperature: 40°C - 45°C
      • Heat inactivation: 70°C / 10 min.
      • Free of endo- and exodeoxyribonucleases, phosphatases, and ribonuclease
      • 200 units/μl
      • High-purity enzyme completely free of E.coli DNA contamination
      • GMP grade
    • Technical Data
      Technical Image
    • Application
      • Gene synthesis
      • First-strand synthesis of cDNA
      • RT-PCR
      • Random priming reactions
      • Library construction
      • Probe labeling
      • mRNA 5’-end mapping
    • Storage & Shipping
      • Store at -20ºC in a frost-free freezer
      • Shipped on ice packs and/or dry ice