| Principle of Procedure |
Real-time PCR |
| Target |
FAM for Cronobacter sakazakii, Cy5 for Internal Control |
| Tests for Package |
96 Tests/kit |
| Reagent Type |
Lyophilized (Freeze-dried) in PCR tube |
| Limit of Detection (LoD) |
2.5 copies/μL |
| Precision |
Respectively detect the reference specimen of high and low concentrations in
different time ranges-10 times, and the precision values of intra-assay and inter-assay
Ct values were all <1.5%. |
| Specificity |
For analytical specificity, cross-reactivity was evaluated by testing similar intestinal bacteria and microorganisms.
The test was repeated three times and showed no cross-reactivity |
| Sample Types |
Enrichment samples, isolate cultures, and microbial cultures. |
| Shipping/Storage |
Shipping and Storage at room temperature for 12 months |
| Compatible Instruments |
CLEO™ Q16 (Wizbiosolutions), CFX96 (Bio-Rad), QuantStudio™ 5 (ThermoFisher) |