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    WizDx™ CrystalMix E. coli O157:H7

    Technical Data Image
    WizDx™ CrystalMix E. coli O157:H7
    WizDx™ CrystalMix E. coli O157:H7 Kit is a research reagent that qualitatively analyzes E. coli O157:H7 from DNA extracted from enrichment samples, isolation cultures, and microbial cultures. Anti-carryover contamination technology is applied to this kit to prevent cross-contamination by repeated PCR tests.
    Catalog Package
    DX1242 96 tests

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    • Overview
      • Simple procedures for E. coli O157:H7 with ready-to-use reagents that assure high specificity & sensitivity.
      • Hot-start PCR technology minimizes non-specific PCR reactions and increases specificity.
      • Uracil-DNA glycosylase (UDG) technology prevents carryover contamination.
      • Ready-to-use freeze-dried PCR mix allows convenient storage and a minimal test procedure.
      • Internal cellular control helps prevent false negatives.
      • Using CLEO Q16, the PCR setup can be easily set up with QR code recognition, and test results can be automatically analyzed based on AI, allowing for quick and intuitive confirmation of results.
    • Specifications
      Principle of Procedure Real-time PCR
      Target FAM for EHEC stx 1, HEX for EHEC stx 2, Cy5 for Internal Control
      Tests for Package 96 Tests/kit
      Reagent Type Lyophilized (Freeze-dried) in PCR tube
      Limit of Detection (LoD) 5.0 copies/μL
      Precision Respectively detect the reference specimen of high and low concentrations in different time ranges-10 times, and the precision values of intra-assay and inter-assay Ct values were all <1.5%.
      Specificity For analytical specificity, cross-reactivity was evaluated by testing similar intestinal bacteria and microorganisms. The test was repeated three times and showed no cross-reactivity.
      Sample Types Enrichment samples, isolate cultures, and microbial cultures.
      Shipping/Storage Shipping and Storage at room temperature for 12 months
      Compatible Instruments CLEO™ Q16 (Wizbiosolutions), CFX96 (Bio-Rad), QuantStudio™ 5 (ThermoFisher)
    • Technical Data
      Technical Image
    • Protocol
      WizDx™ CrystalMix E. coli O157:H7
    • Storage & Shipping
      • Room temperature (4 - 25℃)