The WizDx™ CrystalMix Mycoplasma kit is a fast, easy, and simple mycoplasma testing method. There is no need to wait long periods of time for traditional analysis or need a specialized laboratory to get fast results. The WizDx™ CrystalMix Mycoplasma kit makes it easy to test for mycoplasma within your facility, allowing you to quickly and accurately verify that your manufacturing process is on track. PRINCIPLES OF THE TEST; WizDx™ CrystalMix Mycoplasma combines all reagents necessary for successful Real-time PCR in a convenient individual aliquot and lyophilized in an 8-strip qPCR tube. Real-time PCR technology utilizes polymerase chain reaction (PCR) for the amplification of specific target sequences and target-specific probes for the detection of 16s rRNA gene in extracted DNA of cell cultures and other cell culture-derived biologicals. The probes are labeled with fluorescent reporter and quencher dyes. Probes specific for Mycoplasma spp. 16s rRNA are labeled with the fluorophore FAM. The probe is specific for the Internal Positive Control (IPC) and is labeled with the fluorophore Cy5 to monitor for PCR inhibition and to validate the quality of the sample and detection result.