WizDx™ CrystalMix Sheep ID kit provides a range of testing options for real-time detection of Sheep species to ensure compliance with regulations on labeling foods and the composition of animal feeds.
PRINCIPLES OF THE TEST;
WizDx™ CrystalMix Sheep ID combines all reagents necessary for
successful real-time PCR in a convenient individual aliquot and lyophilized
in an 8-strip qPCR tube. Real-time PCR technology utilizes polymerase
chain reaction (PCR) for the amplification of specific target sequences
and target-specific probes for the Sheep. The UDG system is applied to
prevent carry-over contamination, and the Uracil DNA Glycosylase
treatment and qPCR (Real-time PCR) are continuously performed in one
tube, so it is highly convenient for the user and can prevent contamination
between samples or amplicons. The probes are Dual-labeled with a
fluorescent reporter and quencher dyes. For each labeled probe, probes
specific for the Sheep labeled with the fluorophore FAM. The probe is
specific for the Internal Positive Control (IPC) is labeled with the
fluorophore Cy5 to monitor for PCR inhibition and to validate the quality of
the sample and detection result.