• Conventional PCR > PCR

    Gradient PCR

    Technical Data Image
    Gradient PCR
    Cat No. Size
    Optima96 364x243x244mm
    Gradient PCR are essential laboratory equipment for researchers who run polymerase chain reaction (PCR) for sequencing, cloning, genotyping, mutagenesis, and many other applications. It features precise PCR annealing temperature gradient to easily optimize PCR assays in a single run.

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    • Overview
      • Six Independent temperature Blocks with imported TEC modules provide precise Temperature control for PCR optimization.
      • Gradient PCR thermal cycler with excellent thermal gradient temperature accuracy.
      • Independent front and rear air inlet and outlet duct design, which allows instruments to be placed close to each other, saving laboratory space.
      • Ultra large fin-type heat sink with intelligent fan speed control technology provides low-noise heat dissipation.
      • Flexible lid: Heated lid automatically adjusts to fit every PCR tube, creating pressure that prevents sample evaporation, to ensure reproducible results in every run.
      • The Lid comes with Intelligent auto-heating off function to reduce non-specific amplification by turning off the heating when the temperature of sample blocks is below 30°C.
      • Faster Heating and cooling Rates (Maximum heating rate >5°C/s, maximum cooling rate >4°C/s).
      • Provides excellent temperature uniformity and the gradient layout reduces edge effect.
      • Comes with USB interface at the front for easy gradient PCR protocol import & export. Also, Device software can be upgrade through USB.
      • Gradient PCR machine comes with Multiple Accounts and Access rights (Administrator, User Accounts, Visitor Accounts).
      • High resolution, 7-inch LCD touchscreen panel for easy operation and monitoring.
      • Touchdown (TD) PCR Mode and Long Range PCR Modes are available.
      • Compatible with universal PCR tubes, PCR 8 strips plates and 96-well PCR plates.
    • Specifications
      Sample Capacity 0.2mL PCR Tubes, 0.2mL 8-tube Strips, 0.2mL 96-well PCR Plate (non-skirted/semi-skirted)
      PCR reaction volume 10-100 µl
      Heating lid temperature 30-110°C
      Max. block heating Rate 5°C/s
      Max. block cooling Rate 4 °C/s
      Temperature uniformity <0.5°C (20s after reaching 95°C )
      Sample temperature range 4°C-100°C
      Maximum temperature difference between blocks 25°C (max. 5°C between two adjacent blocks)
      Max. number of steps 55
      Time increment / decrement 1-120s, for long Range PCR experiment
      Temperature increment / decrement 0.1°C-10.0°C, for Touchdown PCR
      Maximum number of cycles 999
      Program storage capacity Up to 1,000 programs in the instrument, Unlimited storage with USB flash drive
      Net weight 8.7kg